Mouse prostaglandin E2 (PGE2) elisa kit, prostaglandin E2 (PGE2) ELISA kit instruction manual This kit is for research use only.
Detection range: 96T
15ng / L-480ng / L

Mouse prostaglandin E2 (PGE2) elisa kit, prostaglandin E2 (PGE2) ELISA kit purpose:
This kit is used to determine the content of prostaglandin E2 (PGE2) in mouse serum, plasma and related liquid samples.
Mouse prostaglandin E2 (PGE2) elisa kit, prostaglandin E2 (PGE2) enzyme-linked immunoassay kit makes the experimental principle of this kit using double antibody sandwich method to determine the level of mouse prostaglandin E2 (PGE2) in the specimen. Microporous plates were coated with purified mouse prostaglandin E2 (PGE2) antibody to make solid phase antibodies. Prostaglandin E2 (PGE2) was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled prostaglandin E2 ( PGE2) The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with prostaglandin E2 (PGE2) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of mouse prostaglandin E2 (PGE2) in the sample was calculated by a standard curve.
Mouse prostaglandin E2 (PGE2) elisa kit, prostaglandin E2 (PGE2) ELISA kit make up the kit
1 30 times concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle
2 Enzyme label reagent 6ml × 1 bottle 8 standard (960ng / L) 0.5ml × 1 bottle
3 Enzyme label coating plate 12 wells × 8 strips 9 Standard diluent 1.5ml × 1 bottle
4 Sample diluent 6ml × 1 bottle 10 Instructions 1 copy
5 Developer A solution 6ml × 1 bottle 11 sealing film 2 sheets
6 Developer B solution 6ml × 1 / bottle 12 sealed bag 1 mouse prostaglandin E2 (PGE2) elisa kit, prostaglandin E2 (PGE2) ELISA kit required for specimen
1. Specimens are extracted as soon as possible after collection, and extraction is performed according to relevant literature, and experiments should be conducted as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided
2. The samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity.
Steps
1. Dilution of standard products: This kit provides one original standard product. The user can perform dilution in a small test tube according to the following chart.
480ng / L No. 5 standard, 150μl original standard, add 150μl standard dilution
240ng / L No. 4 standard 150μl No. 5 standard added 150μl standard dilution
120ng / L No. 3 standard 150μl No. 4 standard added 150μl standard dilution
60ng / L No. 2 standard 150μl No. 3 standard added 150μl standard dilution
30ng / L No. 1 standard 150μl No. 2 standard added 150μl standard diluent

2. Sample addition: set up blank wells (the blank control wells do not add samples and enzyme reagents, the rest of the operation is the same), standard wells, sample wells to be tested. Accurately add 50μl of the standard on the enzyme-coated plate, add 40μl of sample diluent to the sample well, and then add 10μl of the sample to be tested (the final dilution of the sample is 5 times) Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, shake gently to mix.
3. Incubation: Seal the plate with a sealing plate and incubate at 37 ° C for 30 minutes.
4. Mixing solution: Dilute 30 times concentrated washing liquid with distilled water 30 times and reserve
5. Washing: Carefully peel off the sealing film, discard the liquid, spin dry, fill each well with the washing liquid, let it stand for 30 seconds and then discard, repeat this 5 times, pat dry.
6. Add enzyme: add 50μl of enzyme label reagent to each well, except blank well.
7. Incubation: The operation is the same as 3.
8. Washing: The operation is the same as 5.
9. Color development: add 50μl of developer A to each well, then add 50μl of developer B, mix gently, and develop at 37 ° C in the dark for 15 minutes.
10. Termination: Add 50μl of stop solution to each well to stop the reaction (at this time the blue will turn to yellow).
11. Determination: Measure the absorbance (OD value) of each well in sequence with the blank air conditioner at zero and 450 nm wavelength. The measurement should be carried out within 15 minutes after adding the stop solution.
The mouse prostaglandin E2 (PGE2) elisa kit and the prostaglandin E2 (PGE2) ELISA kit summarize the operating procedures:

Calculate the standard concentration as the abscissa and the OD value as the ordinate. Draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or use the standard The linear regression equation of the standard curve is calculated from the concentration and the OD value, the OD value of the sample is substituted into the equation, the sample concentration is calculated, and then multiplied by the dilution factor, which is the actual concentration of the sample.
Precautions
1. The kit should be equilibrated at room temperature for 15-30 minutes before being taken out of the refrigerated environment. If the enzyme label coated plate is unopened, the strip should be stored in a sealed bag.
2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing.
3. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples.
4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the test substance in the specimen is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please first dilute it with a certain multiple (n times) of the sample diluent and then determine it. When calculating, please multiply the total dilution Multiple (× n × 5).
5. The sealing film is limited to one-time use to avoid cross-contamination.
6. Please keep the substrate away from light.
7. Strictly follow the instructions, and the test results must be determined by the microplate reader.
8. All samples, washing liquids and various wastes should be treated as infectious agents.
9. The components of different batches of this reagent shall not be mixed.
10. If there is any difference with the English manual, the English manual shall prevail.
Storage conditions and validity period
1. Kit storage :; 2-8 ℃.
2. Validity: 12 months

Mouse prostaglandin E2 (PGE2) elisa kit, prostaglandin E2 (PGE2) enzyme-linked immunoassay kit are available from stock, please call to order 13764022678

1,Regular basket in various sizes and shapes,all with top quality indonesia cane, no sugar content,no chemical whitener, safe to your bread with FDA certificate.

2.Top quality indonesia cane with featured replaceable patterned wood bottom. The pattern can be customized,each piece of bread is with your heart.

Kitchen-Bread Proofing Basket

bread proofing,bread shaping,kitchen basket

MIANYANG BIGANT TECHNOLOGY CO LTD , https://www.bigantech.com