Cell attachment and growth
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September 22 12:06:50, 2024
The attachment and growth of cells are on artificial attachments, and most of the vertebrate cells cultured in vitro grow in a single layer; therefore, the attachments must have a suitable charge suitable for cell attachment or at least suitable for adsorption of attachment factors. Promotes cell attachment and spreading. Although hematopoietic cell lines, rodent ascites tumor cells, and a few other select cell lines, such as human small cell lung cancer [Carney et, al., 1981] can grow spontaneously under suspension conditions; however, many transformations Cell lines can also be grown under suspension, so their growth does not depend on the surface charge of the attachment. In fact, these cell lines can reproduce on a hydrophobic surface. However, most normal cells need to be in the attachment before they proliferate. Upper spreading [Folkman and Moscona, 1978; Ireland et al., 1989; Danen and Yamada, 2001], which can inhibit cell proliferation when the attachment is not good or excessively dense to cause insufficient spreading. Cells that require attachment to grow are called Anchorage dependent cells. Transformed cells often exhibit anchorage inde-pendent, and they can grow in suspension under shaking culture conditions. Long, or grow on semi-solid medium, such as soft agar.