The United States developed the world's first high-throughput mass spectrometer for proteomics
Researchers from the Pacific Northwest National Laboratory (PNNL) of the US Department of Energy (DOE) have invented a new instrument that can improve the speed and quality of protein expression detection. This protein mass spectrometer (PROMS) is the first high-throughput mass spectrometer developed for proteomics research. This mass spectrometer was adapted by Richard D. Smith and his colleagues from a 9.4 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometer-this resonance mass spectrometer was developed by Bruker Daltonics ( Manufactured by Brooke Dalton, a famous instrument company). According to the PNNL report, PROMS is more than 100 times more sensitive and 10-100 times faster than other MSs. This new design brings together an invention by Smith and his team: dynamic range enhancement applied to massspectrometry (DREAMS) on MS. This overcomes the limitation of the number of molecules that MS can measure at the same time. Using DREAMS researchers can eliminate the impact of the largest and most abundant protein and focus on low-level proteins. Smith said that the smaller the number in biological processes, the more common the "player" plays an important role, just like the role of signaling factors in the process of cell signaling. PNNL researchers used this system to detect a cytomegalovirus, known to cause inflammation of the retina, and three microorganisms of interest to DOE. Using PROMS in 2002, researchers identified more than 1900 proteins (about 3,200 in total, accounting for 61%) of the proteome in Deinococcus Radiodurans (MS Lipton et al., "Global analysis of the Deinococcus radiodurans proteome by usingaccurate mass tags, "Proceedings of the National Academy ofSciences, 99: 11049-54, Aug. 20, 2002.), this result is the most comprehensive proteome analysis result of any organism at that time. John Wronka, vice president of Bruker and general manager of life science systems, believes that PROMS technology is a technology prepared for mammalian proteomics and "metabolomics" (a new metabolic study). At present, PerkinElmer, Bruker and other companies have There are PROMS instruments, and the features of PEI's proteomics mass spectrometry sample preparation workstations include: The fully automatic sealing system eliminates external pollution introduced by manual operations and the environment, and ensures sample recovery and reproducibility. Completely complete the process of sample desalination, concentration and flooding to MALDl tarqet. The sophisticated liquid handling system can precisely control the adsorption and desorption kinetics of the sample, improve sample recovery and reproducibility, and ensure the sensitivity of mass spectrometry detection. 96 samples are processed at a time. Sensitivity: 200fmoI BSA. Millipore Zip Tip or Amika ProTip can be used. Compatible with all commonly used MALDl targets. White Skin Mask,Brightening Skin Mask,Arbutin Mask,Brighten The Skin Mask Guangdong Yueji Cosmetics Co., Ltd , https://www.mask-family.com